动物造模,去卵巢法骨质疏松树鼩模型 z-bio 2021-12-27 438

　　Objective: To replicate the model of human postmenopausal osteoporotic tree shrews through surgical ovariectomy on both sides.

　　Method: According to the "Guidelines for the Clinical Diagnosis and Treatment of Primary Osteoporosis" (hereinafter referred to as "Guidelines") published by the Ministry of Osteoporosis and Bone Mineral Diseases, 100 female trees were tested. The 2011 Chinese Medical Association model evaluation: (1) Trees estimate the peak age of BMD based on natural life span, sexual maturity time, peak bone mineral density (BMD) age, natural life span and sexual maturity time of trees. Randomly select trees with 6, 6, 12, 18, and 24 months old for systematic BMD detection, and compare the differences in BMD in each age group to determine the maximum monthly age of the tree. ② Randomly select monthly peaks. The BMD of 62 BMD ages of the remaining trees sh was tested to determine the average and standard deviation of the BMD peaks of the tree sh. Then, we randomly divided them into a model group (n = 32) and a blank group (n = 30). The surgical group was excised from both sides of the ovary, and the blank group was excised in the same volume as the greater omentum. ovary. In June, September and December, 6 animals were randomly selected for BMD testing, and animals with BMD values that met the “guided” diagnostic criteria were selected for pathological testing and verification.

　　Results: (1) The peak BMD of the tree sh tested at 6, 12, 18 and 24 months old were (0.138±0.012), (0.143±0.010), (0.141±0.009) and (0.139±0.009) grams, respectively There is no significant difference in the peak BMD of tree B at 1 month old (P\→0.05); (2) the average peak BMD of tree sh is (0.143±0.016) g/cm2; (3) the BMD value of the model, the The group meets the diagnostic criteria of the guidelines. The number of sh was 0, 5, 4, and 5, and the pathological section of the proximal tibia was compared with the sh of a 6-month-old tree. And the distance between the trabeculae is enlarged; the BMD value of the blank group is the same at each time point, the number of trees sh for the ``guidance'' diagnostic criteria is 0, and the pathological section of the proximal tibia is A 6-month-old tree sh .

　　Conclusion: According to the diagnostic criteria of the "Guide", a 6-month-old female tree sh can replicate a human postmenopausal osteoporosis model 6 months after bilateral ovariectomy. The model has a high success rate and stability.