动物造模,转基因小鼠模型 z-bio 2021-04-22 389

　　Objective: To observe the expression of Pin1 in the skin and construct a transgenic mouse model that can induce the expression of Pin1 in the skin.

　　Method: clone the mouse Pin1 gene into a modified pTRE2 vector that can be fused with Myc tag protein, and microinject the linearized DNA to construct TRE-Pin1 mice.

　　Result: We successfully obtained the first transgenic mouse of TRE-Pin1. By combining this mouse with an epithelial-specific K14-rtTA transgenic mouse, we obtained a double transgenic mouse that specifically induces Pin1 expression in skin epithelial cells. By adding (called doxycycline) to drinking water to induce the expression of Pin1 gene, Western blot and immunohistochemistry showed that Pin1 protein may be specifically overexpressed in skin epithelial cells. It was also found that endogenous Pin1 is mainly expressed in skin epithelial cells.

　　Conclusion: We have successfully constructed a transgenic mouse model that induces Pin1 expression in the skin.