动物造模,中重度脓毒症 z-bio 2021-04-22 510

　　Objective: To establish a long-term survival rat model of mid-to-severe sepsis, observe the natural course of sepsis, and provide new ideas and methods for the study of sepsis.

　　Method: Forty SD rats were divided into a control group (8 in the sham group) and a small intestine puncture group (32 in the CLP group of cerebellar puncture). All operations were performed using a small animal anesthesia machine and sevoflurane inhalation. anesthesia. All rats have established arteriovenous access to the right neck. After recovery 24 hours after the operation, rats in the CLP group suffered from sepsis due to cecal ligation and perforation. After the operation, the rats were transferred to the recovery room (room temperature 22-25°C) and kept in a cage. On the 1st and 2nd day after operation, 6% HEAS and 5% glucose were supplemented intravenously at a supplementary dose of 1:1 to 20 mL/kg/12h. After that, the mice ate until the water was reduced by half. According to the survival rate of the rats, the rats in the CLP group are naturally divided into a survival group (survival group) and a death group (death group). Patients will be observed 30 days after surgery, IL-10 symptoms, weight changes and plasma concentration will be recorded, and changes in abdominal organs will be observed through anatomical structures.

　　Results: (1) In the CLP group, the survival rate was 75% within 24 hours, 62.5% within 72 hours, and 50% within 7 days. (2) According to the sepsis severity assessment system, 32 rats in the CLP group reached moderate to severe sepsis 24 hours after surgery. (3) After the operation, the weight of the survival group and the sham group decreased. The rats in the survival group decreased significantly from day 4 (P = 0.017). The surviving group had the lowest body weight 6 days after the operation, which was (8.51±2.23)% lower than the original body weight. The sham operation group had the lowest weight 4 days after surgery (2.73). The group difference was statistically significant (P = 0.026) lower than the original body weight by ±1.82)%. Thirty days after surgery, there was no significant difference in the maximum weight gain of the two groups of rats (sham group (16.16±2.39)% and survival group (13.03±3.74)%) (P = 0.29). 4) Compared with the preoperative (0d), the plasma concentration of IL-10 in the three groups all increased, and the survival group (P = 0.000) and the death group (P = 0.010) increased significantly. See abdominal dissection: a large amount of foul-smelling ascites in the death group, the ligated intestine was purple and black, unwrapped, and no adhesions. Connects the intestines, but does not wrap adhesions. There was no abnormal anatomy in the sham group. The spleen of rats accounted for (2.64±0.37)‰ of the body weight, and the spleen of rats in the sham group accounted for (1.63±0.20)‰. The difference between the two was statistically significant (P = 0.032).

　　Conclusion: This experiment established a CLP model for long-term survivors of moderate to severe ascites, and provided a reliable method for controlling CLP and a model screening method.