动物造模,大鼠痛风性肾病模型 z-bio 2022-10-06 590

　　Objective: To study the effects of different doses of yeast, adenine and different modeling periods on the rat gouty nephropathy model.

　　Methods: The male SD rat model group was given oral yeast powder 15 g/kg, adenine 50 mg/kg, and adenine 100 mg/kg. The model days were 8, 10, 22, and 48, respectively. .. Compare the size of the renal hypertrophy index of each group to detect rat renal function, urine index, renal cortex β-N-acetylglucosaminidase (NAG) content, liver xanthine oxidase (XOD) activity and rats Pathological changes. Changes in the kidneys. The expression of NGAL, OAT1 and TIMP-1 in kidney tissue was detected by immunohistochemistry.

　　Results: The increase of the concentration of the model group and the increase of the model construction time made the pathological changes of the kidney more obvious. Model 100 mg/kg adenine and 15 g/kg yeast together for 8 days. Compared with the normal control group, the pathological changes of the kidneys in this group were obvious. Inflammatory cells infiltrated and swelled renal tubular epithelial cells, and the structure of renal tubules and glomeruli was damaged. No; renal hypertrophy index was higher than that of the normal control group (P\u003c0 .01); The renal function groups of models A, B, E and F were lower than the normal control group (P\u003c0.01). The 24-hour urine volume and urine protein content of rats in groups B, E and F and F increased (P\u003c0.01). The expression of NGAL and TIMP-1 in the kidney tissue of E group was higher than that of the normal group (P\u003c0.01), and the expression of OAT1 was lower than that of the normal group (P\u003c0). 01).

　　Conclusion: 100 mg/kg adenine and 15 g/kg yeast for modeling for 8 days is an ideal model dose and model period. GAL, TIMP-1 and OAT1 proteins can affect the kidney damage in rats with severe nephropathy.