动物造模,腹泻型肠易激综合征模型 z-bio 2022-01-14 368

　　Purpose: Combined with capsaicin tube feeding, observe the symptoms, histopathology, visceral hypersensitivity, mast cell activation, autophagy, beclin-1 and claudin-2 and other related indicators changes in rats, evaluate restraint pressure, and establish diarrhea-type intestines New research on irritable syndrome (D-IBS) model.

　　Method: Forty male SD rats of clean grade were divided into normal group, group I, group II and group III according to their body weight, with 10 rats in each group. In the model group, the binding tail clamp stress was combined with capsaicin (0.125% in group I, 0.250% in group II, and 0.500% in group III), and a D-IBS model was established by intragastric administration. Inject the same amount of normal saline into the stomach twice in a row. Powerlab was used to detect the number of rat abdominal wall contractions and dorsal arches, mast cell staining (Fuxin-Orange G method) was used to detect the activation of mast cells, and optical and electron microscopes were used to examine the pathology and autonomy of the rat’s colon. Immunohistochemistry SABC was used to detect the changes of phage to detect the changes in the expression of beclin-1 and claudin-2 in the colon mucosa of rats.

　　Result: All rats in the model group III died, and the fecal count of the rats in the model group II increased and the visceral sensitivity threshold decreased. Compared with the normal group and the model group I, the difference is significant (P\u003c0.05); the shape of the colon mucosa, mucosal epithelial villi and glands of each group of rats are normal, submucosal vasodilation and diffuse inflammation The infiltration did not change; except for the normal group, the mucosal matrix or submucosal tissue of the colon tissue, the model groups I and II were round purple. Until dark purple staining, indicating that the expression of mast cells increased. Compared with the normal group and the model I group, the number of rats in the model II group increased significantly, and the difference was statistically significant (P\u003c0.05).

　　Conclusion: The rat D-IBS model established by the combination of restraint stress and 0.25% capsaicin forced feeding showed increased diarrhea, visceral irritable bowel syndrome, increased mast cell expression, increased intestinal epithelial cell autophagy, and destruction of the intestinal mucosal barrier. IBS, this method is simple, easy to implement, and worthy of widespread use.