动物造模,低血糖模型 z-bio 2022-01-07 366

　　Objective: To establish a rabbit hypoglycemia model and evaluate the accuracy and timeliness of continuous subcutaneous blood glucose monitoring system (CGMS) to monitor hypoglycemia.

　　Method: 16 female New Zealand white rabbits were randomly divided into 4 groups, each with 4 rabbits. The control group received continuous intravenous injection of normal saline, and the experimental group received continuous intravenous injection of insulin. According to different doses, they are divided into insulin 0.1U/(kg·H) group (RI = 0.1U group) and insulin. 0.2U/(kg·H) group (RI = 0.2U group) and insulin 0.4U/(kg·H) group (RI = 0.4U group). During the test, CGMS was monitored for 240 minutes, blood was collected from the ear vein every 30 minutes, and the blood glucose level (BG monitoring value) was monitored using a portable blood glucose meter.

　　Results: During the investigation, 1296 CGMS monitoring data were obtained, and 136 BG monitoring data matched the CGMS time. After insulin administration, BG and CGMS were significantly reduced. The reduction rates of BG and CGMS in the I = 0.1U group were 0.016 and 0.017 mmol/L/min, respectively, and the reduction rates of BG and CGMS in the RI = 0.2U group were 0.04 and 0.027 mmol/L/min, respectively. Minutes; I = 0.4U group were 0.049 and 0.032 mmol/L/min, respectively. According to whether the BG monitoring value is less than 4.4 mmol/L, BG-CGMS data can be divided into two types: hypoglycemia and normoglycemia. The average deviation of BG-CGMS during hypoglycemia is 0.55 mmol/L (upper and lower limit: -0.98? 2.08 mmol/L), the absolute difference (RAD) is 40.2%±45.2%, and the average deviation of BG-CGMS is normal blood glucose level It is -0.19 mmol/L (upper and lower limit: -1.38 to 1.00 mmol/L), and RAD is 5.8% ± 5.3%. Error grid analysis (EGA) shows that area A is 93.4%, area B is 0.7%, area D is 5.9%, and area D is distributed in areas with low BG and high CGMS.

　　Conclusion: The results of this study show that CGMS has a significant hysteresis when the blood sugar level drops faster. When blood sugar drops below 4.4 mmol/L, CGMS may overestimate blood sugar. This risk should be fully considered in the clinical application of CGMS.