肝切除术后急性肝功能衰竭模型,动物造模 z-bio 2021-04-26 271

　　Objective: To explore the effect of different proportions of hepatectomy volume on acute liver failure in Bama mini-pigs, and to provide a suitable method for establishing a suitable model of acute liver failure after most hepatectomy in mini-pigs.

　　Methods: 75%, 85%, and 95% liver resections were performed respectively. The residual liver was examined by CT and the survival status was recorded. Blood was drawn regularly before operation, 1, 3, and 5 days after operation, and 1, 2, and 3 weeks after operation to test liver function. , Obtain HE staining of liver tissue, and check liver pathology.

　　Results: The average survival time of 75%, 85% and 95% hepatectomy minipigs was (19.0±5.6) days, (17.3±5.5) days, and (1.3±1.5) days. Pathology of Bama minipigs with different liver resection ratios The scores were (5.67±0.52), (8.17±0.82), (8.50±0.71). As the proportion of liver resections increases, the incidence of liver failure increases. 85% of liver resection can cause alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, and total bile acid levels to increase significantly.

　　Conclusion: 85% volume hepatectomy can cause a typical model of acute liver failure in miniature pigs.