动物造模,阿尔茨海默病转基因模型 z-bio 2021-04-26 320

　　Purpose: Tg2576 transgenic mice showed pathological changes similar to Alzheimer's disease (AD). This article dynamically studied the characteristics of the serum metabolites of Tg2576 mice at various stages of AD, and provided an early diagnosis for the clinical basis of AD metabolism.

　　Method: Collect serum samples of Tg2576 mice in the early (6 months) and final (12 months) stages of AD onset, collect 1 HNMR spectra of the samples, and collect metabolic characteristics using multivariate analysis techniques.

　　Results: The results showed that the serum metabolic characteristics of Tg2576 and C57 mice were significantly different at 6 months and 12 months, respectively, and there were significant metabolic differences between Tg2576 mice at different stages of AD. In the early stages of AD development, compared with C57 mice, Tg2576 mice have increased serum lactic acid, inositol, amino acids (leucine, isoleucine, alanine, etc.) levels, lipids and choline, The content of phosphatidylcholine/glycerol phosphatidylcholine and betaine is increased. Done. , Glycine and glucose content decreased; at the end of AD attack, serum lactic acid, inositol and alanine content continued to increase, lipid, choline, phosphatidylcholine/glycerol phosphatidylcholine, betaine and glycine content although Continued to decline, but the content of glutamate and creatine initially showed a downward trend. Comparing the serum metabolites of early and late AD shows that serum lactic acid, inositol, and alanine levels increase, and lipid, choline, phosphatidylcholine, and glycerophosphatidylcholine levels decrease in advanced disease. Among these metabolites, lactic acid, lipids, choline, phosphatidylcholine and glycerol phosphatidylcholine have significant changes in the early stages of AD, and are closely related to the severity of AD.

　　Conclusion: The results show that lactic acid in Tg2576 mice is positively correlated with the progression of Alzheimer's disease, while lipid, choline, phosphatidylcholine and glycerophosphatidylcholine are negatively correlated.