动物造模,胶质瘤原位移植瘤模型 z-bio 2021-04-26 379

　　Objective: To establish a stable, real-time monitoring model of glioma allograft in nude mice.

　　Method: U251 glioma cells were infected with lentivirus containing luciferase-Luc and green fluorescent protein-GFP genes, and cell lines stably expressing GFP-Luc fluorescence were screened by flow cytometry to make the fluorescent cells proliferate and change Migration and invasion capabilities. Through CCK-8 experiment, cell cycle experiment, Transwell tumor migration and invasion experiment, cells were inoculated into the caudate nucleus of nude mice to establish an orthotopic glioma transplantation tumor model, and the mouse real-time imaging system was used to monitor tumor growth. The pathological features and tumorigenicity of brain cells in nude mice were evaluated by brain, paraffin sections and HE staining.

　　Result: We successfully constructed a U251 glioma cell line and animal model that stably express GFP fluorescence and luciferase fluorescence. Lentiviral integration does not change the cell's ability to proliferate, migrate or infiltrate. This model has a medium growth cycle and high tumor incidence. , The tumor grows stably in the skull, and the HE film conforms to the characteristics of human glioma.

　　Conclusion: Compared with traditional cells, dual-fluorescence-labeled glioma cells can be used for experimental studies of glioma animal models; U251-GFP-Luc glioma cells have similar tumor growth and human nude mouse models. Pathological characteristics. Glioma is similar, and tumor growth can be observed in real time, making it an ideal animal model for experimental research on glioma.