动物造模,慢性非细菌性前列腺炎 z-bio 2021-10-08 559

　　Objective: To establish a chemical animal model of chronic non-bacterial prostatitis, and to provide reliable model animals and methods for evaluating the etiology and drug research of CNP.

　　Method: The SD male rats were randomly divided into control group and model A, B, and C groups, and 1% sterile carrageenan was injected into the left and right ventral lobes of model A, B, and C groups, respectively. Inject 20, 50, 100 μL of prostate; 50 μL of sterile saline into each control. From the anatomical point of view, treatment 7d, prostaglandin index, white blood cell detection, pathological section and tumor necrosis factor-α (TNF-α), nuclear factor-κB (NF-κB), IκB kinase (IKKα), phosphorylation from five Analyze the expression of IKB-α (p-IKB-α) and cyclooxygenase-2 (COX-2) proteins in several aspects.

　　Result: Compared with the sham operation group, the prostate tissues in models A, B and C are less flexible, less elastic and adhere to surrounding tissues. Prostate index and total white blood cell growth rate increased significantly (P\u003c0.01). The prostate index growth rate of group A and group C were 21.1%, 61.7% and 72.7% respectively; the growth rate of total white blood cell increased to 75.0%, 103.6, The pathological results showed that the prostate stroma in group A was less swollen, while the prostate in groups B and C were significantly swollen. The prostate in group C was significantly atrophy and some glands were degenerated and necrotic; the expression levels of NF-κB in each group, A, B and C IKKα, p-IKB-α, TNF-α and COX-2 in prostate tissue are different. As the expression degree increases, the inflammatory pathway of NF-κB is activated.

　　Conclusion: Injecting 20, 50, 100 μL of 1% carrageenan into the left and right ventral lobes of rat prostate can induce different degrees of prostatitis, but the dose of 20 μL is too low, the inflammation is weak, and major surgical errors are caused; 100 μL of inflammation is too large. It is strong enough to kill some animals; the animal model of prostatitis caused by the 50μL dose is the most successful, and clearly follows the NF-κB inflammatory signal transduction pathway.