动物造模,肌肉萎缩模型 z-bio 2021-12-30 304

　　Objective: Establish an animal model of cynomolgus monkey muscle atrophy, and establish a model evaluation method by restricting the movement of the lower limbs?

　　Method: Use a polymer fiber bandage to fix the right lower limb (from ankle to knee). The cynomolgus monkeys were weighed for 15 weeks. Do you weigh once every two weeks? Around the calves on both sides? Lower limb volume, model 11-13? Perform gastrointestinal MRI on both lower legs for 15 weeks and biopsy of muscles for 15 weeks?

　　Results: 15 weeks after modeling, there was no significant change in animal body weight. The volume around the right cynomolgus monkey, around the right thigh and the right lower limb were significantly lower than before modeling (P) u003c0.05); after 15 weeks of modeling, serum creatinine Blood sugar, blood sugar and alkaline phosphatase were all significantly reduced (P\u003c0.05), and serum cholesterol levels were significantly increased (P\u003c0.05); MRI showed atrophy of the right stomach and small intestine, and compared with before modeling, The volume of the right stomach test is reduced. Muscle biopsy showed that the right gastric fistula was atrophied and the muscle fibers became thin; did the cross-sectional area of the gastrostomy fiber decrease?

　　Conclusion: The muscle atrophy model was established by external fixation to limit the movement of the lower limbs of cynomolgus monkeys. The modeling cycle is appropriate and the operability is strong. It combines around the calf, lower extremity drainage, biochemical indicators, MRI imaging and muscle biopsy.