动物造模,腰椎腹侧神经根牵拉模型 z-bio 2021-12-09 472

　　Purpose: To study the etiology and drug development of amyotrophic lateral sclerosis, establish a rat model of the fourth lumbar and abdominal root traction, and use brain-derived neurotrophic factor as a positive drug to verify the effectiveness of the model. Do you want to use it?

　　Method: First, 5 male SD rats were operated on. One week later, using anti-choline acetyltransferase antibody for immunohistochemical staining, changes in the number of motor neurons in the anterior horn of the spinal cord were observed. 7-week-old male SD male rats were randomly divided into 4 groups, 2 model control groups and 2 brain-derived neurotrophic treatment groups (prevention group immediately after surgery and treatment group 1 week after surgery), and Make changes. How to observe the number of motor neurons by immunohistochemical staining?

　　Result: The rat recovered well after the operation. Is the clinical observation normal? The staining results showed that the motor nerve of the spinal nerve root was obvious after surgical traction. Meta degeneration and death? In the control group, the nerve root traction animals treated with brain-derived neurotrophic factor achieved good therapeutic effects regardless of preventive or therapeutic administration one week after surgery. The number of neurons stained with choline acetyltransferase increased significantly (P\u003c0.0001), and the result was 17.85% vs. 93.06%; 26.6%, compared to 87.27%?

　　Conclusion: The abdominal root of the fourth lumbar vertebrae was successfully established in rats. Is the traction model amyotrophic lateral sclerosis?