动物造模,咳嗽变异性哮喘模型 z-bio 2022-01-22 499

　　OBJECTIVE: To establish a simple animal model of cough and asthma using Ovalbumin Brown (OVA) to sensitize Norwegian (BN) rats.

　　Method: 36 BN rats were divided into three groups: normal control group, model control group and administration group. On the first day, the rats were injected intraperitoneally with 2 mg egg protein and 100 mg Al(OH)3 gel. After 3 weeks, the rats were again injected intraperitoneally with 0.01 mg OVA and 100 mg Al(OH) 3 gel. The normal control group is a rat. The same amount of saline was injected. After 3 weeks, the model control group and the treatment group were sprayed with 1% OVA every other day, and the normal control group was sprayed with physiological saline every 7 days. On the day of spraying, the administration group was given intragastric administration, and the other two groups were given the same amount of normal saline every day for a total of 14 times. Twenty-four hours after the last dose, a capsaicin cough and lung function test (induction test) was performed.

　　Result: Compared with the normal control group, the cough of the model control group was significantly increased (P\u003c0.01), and the total airway resistance (RL) was significantly increased (P\u003c0.05). Compared with the model control group, Cdyn) was significantly reduced (P\u003c 0.05); the number of coughs in the treatment group was significantly reduced (P\u003c0.05), RL was significantly reduced, and the number of coughs was reduced (P\u003c0.05) ). ) And Cdyn increased significantly (P\u003c0.05).

　　Conclusion: This animal model is similar to various clinical features of cough and asthma, and is easy to operate, so it can be used as an animal model of cough and asthma.