动物造模,创伤后应激障碍模型 z-bio 2022-01-31 529

　　Objective: To establish a model of post-traumatic stress disorder through single long-term stress (SPS) and observe the effect of orphan feeding on SPS model rats.

　　Method: 36 rats were randomly divided into normal group, SPS model group and SPS complex in vitro group. Use SPS to model the SPS model group and the SPS composite isolation group, and then combine and model the SPS and the isolation group. Breed alone. Seven days after modeling, measure the weight gain of the rats, the total distance of movement, the crossover and maximum distance of an exercise in a field test, and the number and time of rigid body behavior in the rigid body behavior test.

　　Result: Compared with the normal rat group, the weight gain and maximum distance of an exercise in the SPS model group were significantly reduced (P≥0.01), and the total movement distance and the number of passes were reduced. Done. Decrease (P\u003c0.05), the number and duration of strict behavioral tests increased significantly (P\u003c0.01); The weight gain of rats in the SPS complex orphan group decreased (P\u003c0).05), and the number of times increased And strict behavioral test time (P\u003c0.05). Compared with the SPS model group, the combination of the SPS field experiment and the orphan group increased the total distance of movement, the number of intersections and the maximum distance of a single movement (P\u003c0.05).

　　Conclusion: This experiment successfully replicated a rat model of post-traumatic stress disorder with a single long-term stress.