动物造模,心肌缺血再灌注损伤模型 z-bio 2021-04-27 487

　　Purpose: To conduct a comprehensive assessment to improve the preparation of acute myocardial ischemia-reperfusion injury (MI/RI) models in rats, and to lay a good model foundation for subsequent MI/RI research.

　　Methods: 36 clean Wistar rats were randomly divided into 3 groups: normal group (normal group), left anterior descending artery (LAD) without intervention, sham operation group (sham group), only threading, without LAD ligation: ischemia re In the perfusion group (MI/RI group), LAD was ligated for 30 minutes and reperfused for 120 minutes. After the rats were anesthetized, the electrocardiogram was recorded continuously, a ventilator was used for tracheotomy, and the rat's LAD was inserted into the open chest cavity. Comprehensive evaluation of the model.

　　Results: The ECG of the MI/RI group showed a significant dynamic change of ST-T; the level of creatine kinase isoenzyme (CK? MB) in the MI/RI group increased significantly after 2 hours; compared with the MI group, MI The levels of creatine kinase isoenzymes in the /RI group were significantly increased. The infarct size increased significantly, the cardiomyocytes in the MI/RI group were degeneration and necrosis, and the inflammatory cells infiltrated extensively.

　　Conclusion: The improved modeling method not only reduces the difficulty of surgery, but also successfully establishes a rat MI/RI model, laying a good model foundation for future MI/RI research.