动物造模,高血压模型 z-bio 2021-04-27 393

　　Objective: How to establish a high-fat and high-salt diet Pamani Buda hypertension model and explore its possible causes?

　　Method: 18 Bamamini male pigs were randomly divided into 3 groups: normal control group (NC)? Are there 6 animals in the high-fat (HF) group and the high-fat and high-salt (HFHS) group? Group C received normal diet feeding, HF and HFHS groups received high-fat diet, high-fat diet and high-salt diet for systolic blood pressure (SBP) and diastolic blood pressure (DBP) for 24 consecutive weeks. Measure with feed. Measurements were taken at 8, 16, and 24 weeks. Within 24 weeks of modeling, blood glucose, blood lipids, liver and kidney function, and plasma endothelin 1 (ET-) were measured. 1) Take renin (renin), angiotensin II (AngII), aquaporin 2 (AQP-2), vasopressin (AVP) and vascular endothelial growth factor (VEGF) and other indicators, and the liver? Histopathological observation of the kidney?

　　Results: The NC group, HF group and HFHS group showed a significant increase after 8 weeks of comparing the model with SBP and DBP, showing a continuous upward trend. The HFHS group was higher than the HF group; at the same time, the model was one week after 24 weeks , The body weight of miniature pigs and the liver and kidney indexes of HF and HFHS groups were significantly increased (P\u003c0.05), and plasma TC, CREA and ET-1 levels were also significantly increased (P\u003c0). .05, P\u003c0.01). On the other hand, the BUN level of the HFHS group increased significantly. Decrease (P\u003c0.05), but renin? expensive? Me AQP-2? Is the AVP content significantly increased (P\u003c0.05, P\u003c0.01)? Oil red "O" staining results indicate liver lipid deposition. Has the cause changed, such as thickening of the kidneys and renal arteries in the HF and HFHS groups?

　　Conclusion: 8 weeks after induction, a mini-pig hypertension model can be established through a high-fat, high-salt diet. Is the etiology possibly related to affecting renal function, activating the RAS system and AVP?