脂肪干细胞,骨髓间充质干细胞 z-bio 2021-04-28 270

　　Objective: To study the ability of adipose stem cells (ASCs) to differentiate into endothelial cells and compare them with bone marrow mesenchymal stem cells (BMSCs).

　　Method: Isolate and culture ASC and BMSC from SD rats, select specific generations of growing cells, and identify cell surface markers by flow cytometry. Use CCK-8 method to draw cell proliferation curve. Use qPCR to detect the expression of endothelial cell-specific markers CD31, KDR, and vWF mRNA for 3 weeks; use immunofluorescence staining to observe the expression of surface antigen CD31; Dil-labeled acetylated low-density lipoprotein (Dil-Ac-LDL) is used to detect expression Induction group cell uptake function; verify the ability of induction group cells to form tubes on Matrigel.

　　Result: The rat ASC and BMSC were successfully isolated and cultured. In terms of morphology, ASC and BMSC are similar in shape, showing long spindle-like, spindle-like and fibroblast-like morphologies. The results of flow cytometry showed that these two BMSCs are ASCs, which are the surface markers of hematopoietic stem cells, high mesenchymal CD29 (100% and 96.9%), CD90 (96.5% and 99.2%) and hematopoietic cells. Surface markers, I expressed some CD45, (3.9% and 0.8%). It is low-expressed and proved to be extracted mesenchymal stem cells. The results of CCK-8 growth experiments show that the growth rate of ASC is faster than that of BMSC (P\u003c0.05). After standard endothelial cell induction, qPCR results showed that the expression of CD31, KDR and vWF mRNA in BMSC and ASC in the induced group was higher than that in the non-induced group (P\u003c0.05). Immunofluorescence staining showed that the CD31 antigen was expressed on the cells. The membrane surface of the induction group; the uptake of cells in the Dil-Ac-LDL induction group under a fluorescence microscope (no uptake in the non-induction group); the cells of the induction group have the ability to form tubes on Matrigel and be induced. These cells are endothelial cells.

　　Conclusion: Both BMSC and ASC can induce endothelial cell differentiation. ASC can be widely differentiated into endothelial cells in a short period of time, and has stronger proliferation ability, and ASC has more uses than BMSC.