下丘脑中肾阳虚 z-bio 2021-04-28 252

　　OBJECTIVE: To further study the components of renal insufficiency in Lewis rats by observing the expression and ultrastructure of genes related to renal insufficiency in the hypothalamus of Lewis rats.

　　Method: Compare the kidney deficiency model of Lewis rats with subcutaneous injection of hydrocortisone to detect TNF-α, IFN-γ, IL-10, CRH, MR and hypothalamus in each group of rats. Observe the upper structure of hypothalamus and adrenal gland to study the expression of GR mRNA and the mechanism of kidney deficiency in Lewis rats.

　　Results: Compared with normal Wistar rats, the expression of GR and IL-2 mRNA in the hypothalamus of Lewis group rats increased significantly (P\u003c0.01), while the expressions of CRH, TNF-α, and IFN- were γ and Hypothalamus-10 mRNA in the IL Lewis group was significantly increased. Decrease (P\u003c0.01); the adrenal cortex bandage of Lewis rats is slightly thin, mitochondria are weakened, slightly deformed and swollen, and nuclei; Lewis rats have a large proportion of hypothalamic neurons with irregular nuclei, and mitochondria are significantly reduced. Nerve cell synapses decrease, and presynaptic secretory vesicles decrease.

　　Conclusion: There are some microstructural changes in the hypothalamus and adrenal glands of Lewis rats. The abnormal expression of immune-related cytokines and CRH mRNA may be related to the functional imbalance of its own HPA axis, and gender exists.