动物造模,IGF-1基因 z-bio 2021-11-01 368

　　Objective: To determine the expression distribution of IGF-1 gene in Tibetan minipigs of different ages and tissues, and to provide basic data for future research.

　　Method: Using real-time fluorescent quantitative PCR technology, using GAPDH gene as an internal reference, we detected the changes in IGF-1 gene expression in different tissues and growth stages of Tibetan miniature pigs.

　　Results: (1) Continuous research on IGF-1 gene expression showed that the expression level of muscle tissue was the lowest at 0, 0.25, 0.5, 1 and 2 years, while the expression level of skin tissue was the highest at 0. I proved it. At 0.25 and 0.5 years, the liver tissue expression level was the highest at 0.1, 2 and 3 years, respectively. (2) IGF-1 gene is expressed in 7 different tissues. At the age of 0.25, its expression is arranged in descending order: skin, lung, liver, kidney, spleen, heart, and muscle. And the highest expression on the skin, and significantly higher than other tissues (P\u003c0.01). At the same time, with age, the expression of IGF-1 gene in each tissue reaches its peak in 0.25 years.

　　Conclusion: The expression of IGF-1 gene in Tibetan mini-pigs has obvious temporal and spatial specificity.