动物造模 z-bio 2023-03-04 645

　　OBJECTIVE: To investigate the body's inflammatory response after deep second-degree burns in sterile rats infected with Staphylococcus aureus, and to establish sterile rats and SPF rats with deep second-degree burns and Staphylococcus aureus after burns. Infectious diseases need to be checked. A model used to analyze the healing process, inflammatory response and pathological changes.

　　Method: 206-week-old female Wistar sterile rats were treated with a constant temperature and constant temperature burn device at 94°C on a clean workbench for 8 seconds, causing 2 deep burns. Randomly divided into two groups. The wound infection concentration of one group was 1 x 108 CFU/mL Staphylococcus aureus 0.5 mL, and the other group was untreated, and the wound remained sterile. Twenty 6-week-old SPF Wistar female rats were cauterized under the same conditions and randomly grouped. The wound infection concentration of the two groups and one group was 1 x 108 CFU/mL. Staphylococcus aureus 0.5 mL, this group was not treated. Observe the wound nodule formation time, dementia time and healing time of the four groups of rats. Serum was collected before burns (0 hours), 24 hours, 48 hours, 3, 5, 7, 10, and 14 days, and tumor necrosis factor-a was detected. (TNF-α), interleukin-1α (IL-1α) and epidermal growth factor receptor (ECFR) changes: 24 hours after burn, HE staining of skin at 34, 7 and 10 days, pathological changes observed

　　Results: The wound healing and healing time of sterile rats infected by Staphylococcus aureus was significantly shorter than that of the other three groups (P\u003c0.05); SPF rats infected by Staphylococcus aureus had knots, its shrinkage, tumor clearance and The healing time was significantly shortened. Longer time (the other three groups (P\u003c0.05); the levels of serum inflammatory factors TNF-α and IL-1α were double-damaged and infected by Staphylococcus aureus. In SPF-grade burn rats, it increased , And significantly higher than the control group. 72 hours after the burn, the other three groups (P\u003c0.05); due to the strong reaction of the infertile rats to the burn and Staphylococcus aureus stimulation, the serum TNF-α, IL- The levels of 1α and EGFR increased rapidly and significantly. Instead of the situation at 24 hours in the two groups. In SPF rats with burns (P\u003c0.05), the serum EGFR levels at 48 hours of burns were significantly higher than Two groups of SPF rats (P\u003c0.05). Pathology showed that the angiogenesis and granulation tissue formation in the wounds of rats infected with Staphylococcus aureus SPF were longer than those of the other three groups; rats infected with Staphylococcus aureus aseptically The time for angiogenesis and granulation tissue formation in the wound is shorter. It is more than other people. Group 3.

　　Conclusion: Since the immune system of sterile rats is in a dormant state, it will be stimulated by burns and Staphylococcus aureus, thereby rapidly activating the systemic immune system and promoting wound healing. SPF burned rats were infected with Staphylococcus aureus. Various antigen escape molecules can aggravate the body's inflammatory response and delay wound healing.