动物造模,小鼠抑郁模型 z-bio 2021-04-29 465

　　Objective: To investigate the correlation between the expression of brain-derived neurotrophic factor and vascular endothelial growth factor in hippocampus, forebrain cortex and depression caused by chronic stress.

　　Method: 58 SPF grade KM mice and 29 male and female mice were selected. The mice were randomly divided into a control group and an observation group. The mice in the observation group were stimulated by various stress factors such as fasting and electric shocks on the soles of the feet. One mouse was randomly selected every day. The mice in the control group No. 20 were not subjected to experimental stressors and were raised under the same conditions. The body weight of the mice was measured before the stress and 10 and 20 days after the stress. The two groups of mice were tested through Morris water maze experiment and field experiment, and the results were observed and recorded. The expression of BDNF and VEGF in the hippocampus and forebrain cortex of each group of mice will be measured, and their correlation will be analyzed.

　　Result: Before the stress, the weight levels of the two groups of mice were not significantly different (P \u003c0.05). After stress, the weight of mice in the observation group was significantly lower than that of the control group (P0.05). The correction time and vertical movement scores of the observation group were significantly lower than those of the control group, and the middle time was significantly longer than that of the control group (P0.05). The expression levels of BDNF and VEGF in hippocampal CA1 area and forebrain cortex of the observation group were significantly lower than those of the control group (P0.05).

　　Conclusion: Chronic stress can cause depression-like behavior in mice. This may be related to the decreased expression of BDNF and VEGF in the hippocampus and forebrain cortex.