动物造模,特异性心肌缺血模型 z-bio 2021-12-24 307

　　OBJECTIVE: To observe the expression of HE staining in myocardial tissue sections caused by subcutaneous injection of isoproterenol for different days (1 mg/kg body weight), and to observe the expression of VEGF (vascular endothelial growth factor) and FCF (fibroblast growth factor). Rat-specific cardiomyopathy caused by changes in electrocardiogram.

　　Method: Except for the blank group, rats in each group were injected subcutaneously with isoproterenol (1mg/kg) at different locations, and subcutaneously injected with isoproterenol (1mg/kg) for 2 consecutive days. Subcutaneous multiple injections are different. Continuous subcutaneous injection of isopurine (1 mg/kg) 4d group and isoproterenol (1 mg/kg) 6d group. One week after the administration, the rats were anesthetized, the electrocardiogram was taken, the heart was taken, and the changes in the degree of damage to the rat cardiomyocytes were observed by HE staining, and the myocardial tissue was observed by immunohistochemical staining. Slice VEGF (vascular endothelial growth factor) and FGF (synthetic growth factor). The expression of fibroblast growth factor is altered.

　　Results: The 6d group of continuous subcutaneous injection of isoproterenol (1 mg/kg) HE stained tissue sections showed significant differences compared with the blank control group under the microscope. At the same time, the myocardial tissue slice VEGF of the model group was expanded as follows: the modeling time was increased. There are also significant differences in (vascular endothelial growth factor), FCF (fibroblast growth factor) expression and the blank control group.

　　Conclusion: The results of this study showed that the 6-d group of isoproterenol (1 mg/kg) of rat-specific cardiomyopathy myocardial tissue sections HE stained, continuous subcutaneous VECF (vascular endothelial growth factor) factor compared with the blank control group . Due to the significant difference between the expression of FGF (fibroblast growth factor) and the changes in the T wave of the electrocardiogram, isoproterenol (1 mg/kg) can be injected subcutaneously into rats for 6 consecutive days as a treatment for specific cardiomyopathy. Evaluation of the efficacy of the drug. it. Use experimental animal models.