高脂血症易感大鼠,动物造模 z-bio 2021-04-29 559

　　Objective: To observe the etiology and pathological characteristics of rats with spontaneous hindlimb paralysis in hyperlipidemia-sensitive rat populations, to preliminarily study its etiology, and to explore its application and scientific research value.

　　Method: In the WSHc rat population raised in our center, 8 rats with spontaneous hindlimb paralysis and 8 rats with the same age but no paralysis were fed with a normal diet and a high-fat diet, respectively. Hyperlipidemia; use magnetic resonance imaging and histopathology to observe central nervous system diseases in different parts of hindlimb paralysis rats; use TUNEL immunohistochemistry to observe the level of apoptosis; use RT-PCR to express caspase to detect central nervous system Various parts of the nervous system. The 1 and IL-1β genes of the nervous system and protein levels were detected by Western blotting.

　　Result: Both male and female WSHc rats with paralyzed hind limbs can suffer from this disease, and the sensitivity to high-fat diet is not significantly different from that of non-paralyzed WSHc rats. Magnetic resonance imaging showed no obvious lesions in the brain. Compared with WSHc rats without hindlimb paralysis, a large number of inflammatory cell infiltration and positive expression of TUNEL, caspase-1 and IL were observed in the middle and back of the spinal cord of WSHc rats with hindlimb paralysis. The -1β gene in the middle and posterior part of the spinal cord increased significantly (P\u003c0.05, P\u003c0.01), and protein expression also increased significantly (P\u003c0.05).

　　Conclusion: Spontaneous hindlimb paralysis in hyperlipidemia-sensitive WSHc rats is a progressive disease that occurs in the middle and back of the spinal cord. The pathological features are inflammatory cell infiltration and neuronal degeneration and apoptosis. The cause is possible.