动物造模,原位移植瘤,肝癌细胞 z-bio 2021-11-15 761

　　Objective: To investigate the inhibitory effect of Saxifraga ethyl acetate extract on mouse liver cancer cells and orthotopic xenograft tumors.

　　Method: In vitro experiments, H22 cells were treated with different concentrations of EST for 48 hours, then AO/EB double fluorescent staining was performed, the cell morphology changes were observed by laser confocal microscope, and H22 cell apoptosis was detected by nucleic acid electrophoresis and flow cytometry. A mouse liver cancer sympathetic xenograft model was established, and in vivo experiments were carried out to compare the tumor inhibition rate, T lymphocyte proliferation ability and liver pathological changes of tumor-bearing mice with low, medium and high doses of EST.

　　Result: After EST treatment, the apoptosis of H22 cells has undergone morphological changes, and it is positively correlated with the concentration. Nucleic acid electrophoresis showed a characteristic ladder of apoptosis. Flow cytometry analysis showed that the apoptosis rate of the 500μg/mL treatment group reached 31%. In vivo experiments showed that the low-dose, medium-dose and high-dose tumor inhibition rates of EST were 33.0%, 46.7% and 64.3%, respectively. This significantly improved the thymus index and proliferation ability of mouse T lymphocytes (P\u003c0.05); histological observations showed that ESTs are different from each other. The cancer cells in the dose group showed different degrees of growth inhibition, morphological cancellous inhibition and reduced infiltration into surrounding tissues.

　　Conclusion: EST can enhance the immune function of mice, induce tumor cell apoptosis, has obvious anti-tumor effect, and is positively correlated with dose.