动物造模,小鼠急性肠炎 z-bio 2021-04-29 277

　　Objective: To investigate the protective effect of camel milk on acute enteritis induced by dextran sulfate sodium sulfate (DSS) in mice.

　　Method: Male C57BL/6 mice aged 6-8 weeks were randomly divided into two groups. The control group was gavage with double distilled water, and the experimental group was gavage with camel milk. After 14 days, drinking 3% DSS water solution for free can induce acute enteritis. The proportion of immune cells in the experimental group and the control group was analyzed by flow cytometry, and the content of cytokines was determined by enzyme immunoassay.

　　Result: Compared with the redistributed water control group, the mice in the camel milk experimental group slowly decreased (P\u003c0.05), the survival rate increased, and the histological score (P\u003c0.). 05), IFN-γ (IFN-γ)) helper T lymphocytes (CD4 +IFN-γ+) positive cell expression rate increased (P\u003c0.05), helper T lymphocytes (CD4 + IL-17 +) cells Percentage positive interleukin 17 (IL-17)) decreased (P\u003c0.05), IFN-γ in the cell culture supernatant increased (P\u003c0.05), tumor necrosis factor-α (TNF)-α ) And IL-17 decreased (P\u003c0.05).

　　Conclusion: Camel milk can reduce DSS-induced acute enteritis in mice by regulating T cell differentiation and cytokine secretion.