动物造模,新生大鼠急性肺损伤 z-bio 2021-04-29 713

　　Objective: To investigate the effect of geraniol on meconium-induced acute lung injury in newborn rats and its molecular mechanism.

　　Method: 48 1-day-old SPF male SD rats were injected with 1.5 mL kg of meconium through tracheal intubation to establish a meconium lung injury model. The rats were randomly divided into four groups: control group, model group, methylprednisolone group and geraniol group. The automatic blood gas analyzer detects the arterial oxygenation index of each group of rats. HE staining is used to detect the degree of pathological damage to the lung tissue of rats and to score the pathological damage of the lung. Twenty-four hours after establishing the lung injury model, the lung tissue was taken out and its quality was measured. Enzyme immunoassay is used to detect inflammatory factors, such as tumor necrosis factor-α (TNF-α) and interleukin-1β. (Interleukin-1β, II-1β) and II-6 levels; using Western blotting, pilin domain 3 (NLRP3) inflammasome, cleavedcaspase-1 (cleavedcaspase-1), C-caspase-1) and L-detected NOD-like receptors include. 1β protein level.

　　Results: Compared with the model group, the mean arterial oxygenation index of rats in the methylprednisolone group and geraniol group were significantly reduced (P both \u003cP \u003cu003c0.05), the degree of pathological damage and lung injury Pathological changes. The target score was significantly reduced (all P\u003c0.05), the levels of inflammatory factors TNF-α, L-1β and -6 were significantly reduced (all P\u003c0.01), NLRP3, c. -The expression of Caspase-1 I-1β protein significantly reduces the regulatory effect (all P\u003c0.01)

　　Conclusion: The change of odor lignin reduces the arterial oxygenation index and the expression level of inflammatory factors, while NLRP3 has a specific therapeutic effect on meconium induced acute lung injury in newborn rats by down-regulating the expression level. , C-caspase-1 and L-1β protein.