动物造模,局灶性脑缺血模型 z-bio 2021-12-27 475

　　Objective: To analyze and evaluate the correlation between three successful suture methods to establish a rat middle cerebral artery occlusion model, and to provide new methods and standards for evaluating the model.

　　Method: A total of (30) male SD rats were randomly divided into a sham operation group (n = 15) and a model group (n = 15). For minor improvements in local brain preparation, see Zea-Longa thread embolization. The ischemia model is used to detect the changes of cerebral blood flow in each group of rats before and after surgery. We set the area of cerebral infarction in rats as the gold standard, and analyzed the correlation between this standard and changes in cerebral blood flow.

　　Results: The proportion of cerebral infarction area in model rats is positively correlated with changes in cerebral blood flow, and has nothing to do with behavioral scores.

　　Conclusion: The change of cerebral blood flow in rats can be used as a criterion for evaluating the success of a model of focal cerebral ischemia.