动物造模,微小骨粒回植 z-bio 2021-05-07 545

　　OBJECTIVE: To establish a rabbit model of bone replantation after opening the window of the lumbar lamina in rabbits, and to provide clinical experimental evidence.

　　Method: Divide 18 New Zealand male rabbits into two groups. In the control group, after general anesthesia, the L5 spinous process was placed in the center, the left L5 lamina was completely exposed, the lamina and ligamentum flavum were slowly bitten with a microgun, and fenestration was performed. The window about the bone was exposed. 0.8x0.3 cm size. Immediately after stitching. After opening the left window of L5, the experimental group cut out a piece of bone bitten by the window, put it into a medical collagen sponge to form an arch bridge, placed it on the epidural window and sutured it. After the operation, observe the CT and histological changes of the opening position of the lamina.

　　Results: According to the results of CT examination, the small bone particles of the experimental group formed a thin bone plate at the opening of the window 8 weeks after the operation. The bone plate formed after 12 weeks was very thick, the trabecula was continuous, and the shape and volume of the spinal canal did not change significantly. The spinal cord is compressed. In the control group, a small amount of bone hyperplasia was observed at the 8th and 12th weeks of opening the window, the surrounding adhesive tissue penetrated the spinal canal, and the spinal canal was not reconstructed.

　　Conclusion: By re-implanting small autologous bone particles into the opening of the rabbit lamina for bone reconstruction, it can prevent scars from invading the spinal canal and reduce spinal adhesions.