动物造模,可诱导性肝损伤模型 z-bio 2021-12-21 606

　　OBJECTIVE: To establish an Alb-cre/DTR double transgenic mouse model, perform related phenotypic analysis, and on this basis, establish an inducible liver injury model for liver disease related research.

　　Method: After breeding the introduced Alb-cre mice and DTR mice, double transgenic mice are obtained by mating. The DNA in mouse tail tissue was extracted and genotyped by PCR. Double transgenic mice were injected intraperitoneally with diphtheria toxin, then weighed and blood samples were collected at different time points to detect serum ALT and AST levels.

　　Result: Alb-cre and DTR mice were bred and screened to obtain Alb-cre/DTR double transgenic mice. The use of 0.625g/g diphtheria toxin in mice can significantly increase the ALT and AST in the serum of mice. After the mice were dissected, the liver was observed to turn white. The results of HE staining showed that liver cells were obviously necrotic.

　　Conclusion: We have successfully established a mouse model that may induce specific liver damage.