动物造模,原位肝移植模型 z-bio 2021-09-29 542

　　Objective: To establish methods and techniques for orthotopic liver transplantation models in sandy soil.

　　Method: 100 cases of Gerville orthotopic liver transplantation were performed using modified double cannulation, portal vein and inferior vena cava main venous cannulation anastomosis, superior hepatic vein suture, and complete bile duct reconstruction with bile duct stent.

　　Results: After the operation is mature, the donor's operation time is 34.56±5.12 minutes, the liver repair and cannula placement time is 15.43±2.75 minutes, the recipient's operation time is 58.37±8.54 minutes, and the anhepatic stage is 23.66, which is ±. The success rate of 4.47 minutes of surgery is 86.67% (survival rate of 6 hours or more after surgery).

　　Conclusion: The improved double intubation method has successfully established a gerbil liver transplantation model, and training in surgical skills can increase the success rate of surgery and prolong the survival time of gerbils.