动物造模,糖尿病模型 z-bio 2021-05-08 474

　　Objective: Combine a high-fat diet with streptozotocin (STZ) to establish a type 2 diabetes model for beagle dogs and observe its related characteristics.

　　Method: 30 normal male beagle dogs were randomly divided into 3 groups: control group, high-fat group, and diabetes model group, each with 10 dogs. The diabetes model group was fed a high-fat diet and STZ was injected at the same time for 2 months, the high-fat group was fed a high-fat diet, and the control group was fed a regular diet for 5 months. The animal's Lee index, fasting blood glucose, insulin and urine glucose were measured regularly, and an OGTT test was performed in March. After that, blood biochemical tests were performed, such as blood lipid tests and histopathological examinations of the liver and pancreas.

　　Results: After a high-fat diet for 2 months, the Lee index of the high-fat group and the diabetes model group increased significantly (P0.05), and the blood sugar level of the diabetes model group was 11.1 mmol/L... Pancreatic tissue disease in OGTT In the test, it did not recover after 3 hours and 3 hours respectively. Physical examination revealed some injuries and illnesses.

　　Conclusion: A high-fat diet can cause insulin resistance and hyperlipidemia in beagle dogs, but it cannot form a hyperglycemic state. However, after mixing a high-fat diet with STZ injection, Beagle dogs developed insulin resistance along with hyperlipidemia. The typical symptoms of human T2DM include insulin resistance, hyperglycemia, hyperinsulinemia and hyperlipidemia.