动物造模,不同急性应激反应 z-bio 2021-05-08 901

　　Objective: To compare the changes of three acute stress response animal models and stress-related indicators, and seek to establish a relatively suitable acute stress response model.

　　Method: 40 healthy adult male SD rats were randomly divided into normal group (I), tail docking group (II), burn group (III) and amputation group (IV), each with 10 rats. In the normal group without treatment, the tail hanging group was cut off the tail shaft near the end of the tail (between the S3 and S4 spinal nerves), and the burn group was used as a burn model for body surface area. The III degree is 30%. The amputation of the left lower limb in the amputation group was considered to be a severe traumatic stress. Enzyme immunoassay was used to detect serum adrenocorticotropic hormone (ACTH) and corticosterone (CORT) levels in each group of rats, and immunohistochemical methods were used to detect the expression level of hypothalamic c-fos protein.

　　Result: Compared with group I, the levels of ACTH and CORT in groups II, III and IV increased on average (P0.05).

　　Conclusion: The acute stress response model created by cutting the tail stem into a low position has simple operating procedures, consistent damage levels and obvious stress effects. It can more objectively reflect people's stress response when encountering unexpected situations. accident. This is an ideal way to model acute stress responses.