动物造模,解脲支原体感染动物模型 z-bio 2021-12-07 422

　　Purpose: To study the advantages and disadvantages of various modeling techniques, and to select the most suitable animal model method to infect Ureaplasma.

　　Method: Select SPF grade BALB/c female mice and Wistar rats, establish a low-dose UU3 bacterial solution for multiple infections, establish a high-dose UU3 bacterial solution for one infection, and then use high-dose estradiol Parabens as an intervention. Animal models infected with Ureaplasma on the 14, 28, 42 and 56 days after the initial vaccination, cervical secretions from laboratory animals were collected for Ureaplasma culture, and genitals were collected for morphological analysis.

　　Results: (1) Compared with Wistar rats, BALB/c mice are in poorer general condition, with increased vaginal discharge and genital ulcers, but there is no significant change in the vulva of Wistar rats. (2) When comparing the rate of vaginal UU colony formation in each group of rats, the difference was statistically significant (P\u003c0.05). The difference between the low-dose estrogen group and the low-dose non-estrogen group was statistically significant (P\u003c0.05). The difference between the estrogen group and the high-dose non-estrogen group was statistically significant (P\u003c0.05). The difference between the low-dose estrogen group and the high-dose estrogen group was statistically significant (P\u003c0.05). There was a statistically significant difference between the low-dose non-estrogen group and the high-dose non-estrogen group (P\u003c0.05). The results of each group of mice are similar to those of rats. ③Under the same intervention, the difference between rats and mice under low-dose estrogen treatment was statistically significant (P\u003c0.05), and the difference under high-dose estrogen treatment was statistically significant (P\u003c0). 05). (U003c0.05), indicating that under the same treatment, the UU colonization rate of mice is higher. ④ Visual observation: Each rat in the low-dose estrogen group had edema in the reproductive tract tissue, and the rats in the rest of the reproductive tract tissue were soft and had good exercise ability. The genital tissues of the blank group were softer. The germline tissues of mice in the low-dose estrogen group were congested, spunlaced, thickened, stiffness and elasticity decreased. The mice in the high-dose estrogen group were mainly loose and edema. Only the vagina and cervix were seen in the low-dose non-estrogen group and the high-dose non-estrogen group. Mild hyperemia and edema. (5) Microscopic image: There was no obvious pathological damage to the vaginal tissue of rats in each intervention group, and no connective tissue loosening and edema. The mice in the low-dose estrogen group had severe pathological damage; the low-dose non-estrogen and high-dose estrogen mice had less congestion and edema, slightly enlarged glands, and inflammatory secretions.

　　Conclusion: Compared with Wistar rats, BALB/c mice have obvious damage to the vulva. Estrogen pretreatment may increase the efficiency of UU plating. Value rate; BALB/c mice have a high UU colonization rate in the vagina; BALB/c mice have severe pathological damage, which is more valuable for animal experimental research.