动物造模,哮喘小鼠模型 z-bio 2021-05-08 252

　　OBJECTIVE: To observe the role of interleukin 22 (IL-22) in asthma models, and to study airway inflammation and budesonide's regulatory effects on IL-22 in asthma models.

　　Method: 24 BALB/c mice were randomly divided into three groups: control group, asthma model group and budesonide group, and the mice were sensitized and stimulated with ovalbumin (OVA) to stimulate the asthma model. Mouse lung tissue was stained with HE and AB-PAS to record airway inflammation. ELISA was used to detect the level of IL-22 in the three groups of mouse alveolar lavage fluid (BALF), and real-time fluorescent quantitative PCR was used to detect the IL in the lung tissue of mice. -22 mRNA expression level.

　　Results: Compared with the control group, the lung tissue inflammation score of asthmatic mice increased, the level of IL-22 in BALF increased, and the level of IL-22 mRNA in the lung tissue increased. This difference is statistically significant. After budesonide treatment, the airway inflammation score and IL-22 mRNA expression in the lung tissue of the mice were lower than those in the asthma group, and the difference was statistically significant.

　　Conclusion: The therapeutic effect of budesonide on airway inflammation in asthma is related to the inhibition of IL-22 expression and lung secretion.