动物造模,间断性禁食模型 z-bio 2021-05-10 296

　　Objective: To study stable and reliable alternatives to traditional calorie restriction and its effect on blood glucose dynamic balance in the establishment of a mouse model.

　　Method: 40 SPF 16-week-old C57BL/6J mice (half male and half male) were randomly divided into 4 groups according to gender and feeding method. The free-eating group received the basic diet freely, while the intermittent fasting group alternated between normal and fasting for 24 hours, and freely drank alcohol on fasting days. The body weight and blood glucose levels of each group of mice were monitored, and the transabdominal glucose tolerance test and the transabdominal insulin tolerance test were performed 12 weeks before and after modeling.

　　Result: After 12 weeks of intermittent fasting, the body weight and fasting blood glucose of the mice did not change significantly. After intraperitoneal injection of glucose, the blood glucose level rose and decreased, and the proportion of blood glucose level decreased after intraperitoneal injection of insulin. Compared with the control group, the area under the drug resistance experiment curve was significantly reduced (P\u003c0.05).

　　Conclusion: After intermittent fasting, mice increased insulin sensitivity and improved glucose tolerance. This modeling method is easy to operate and is an effective alternative to the traditional calorie restriction model.