动物造模,肠系膜上静脉血栓 z-bio 2021-12-22 434

　　Objective: To establish a rat model of superior mesenteric vein thrombosis by ligation, to simulate the pathological process of the disease, and to provide basic preparations for the study of its etiology and treatment.

　　Method: 96 male SD rats were randomly divided into 3 groups, namely group A (sham operation group), group B (strain group) and group C (simple group), each with 32 rats. The rats in group A only opened the abdominal cavity without interrupting the blood supply. Rats were sacrificed in batches at 8, 24, 48 and 72 hours after the operation. Rats in group B and C were ligated to establish superior mesenteric vein thrombosis and strangulation. At 8, 24, 48, and 72 hours after modeling, simple rat models were sacrificed in batches. HE staining is used to observe the morphological changes and score damage of rat intestinal tissues. ELISA is used to detect the levels of rat serum midgut fatty acid binding protein (IFABP) and α-glutathione S transferase (α-GST).

　　Results: HE staining and pathology scores showed that compared with group A, rats in group B and group C had different degrees of intestinal tissue congestion and damage, group B gradually aggravated, and group C gradually decreased. The degree of blood stasis and injury was positively correlated with the degree of ligation. The results of the serum ELISA method showed that compared with group A, the levels of IFABP and α-GST in the serum of rats in groups B and C were different (P\u003c0.05), and the degree of increase was different.

　　Conclusion: In this study, we successfully established a rat model of superior mesenteric vein thrombosis using ligation. It is simple and easy to perform, has a high success rate of surgery, and can be used for related research.