动物造模,肿瘤模型 z-bio 2021-05-10 612

　　Objective: To study the tumor-targeting properties of farnesyl thiosalicylic acid (FTS) and heptamethylamine carbocyanine (Near-infrared, NIR) fluorescent dye binding compounds and their application in in vivo imaging.

　　Method: Culture human breast cancer cells MCF-7, glioma cells U251 and prostate cancer cells PC3 in the logarithmic growth phase, and then add different concentrations of FTS and FTS-IR783 to prepare two compounds for tumor cells. Inhibition; Add FTS-IR783 (20μmol/L) to 3 cultured tumor cells, observe the aggregation of fluorescent dyes in tumor cells under a fluorescence microscope; 3 tumor cells (each 1×106) are exposed for two weeks. After subcutaneous transplantation, FTS-IR783 (each 10nmol/L) was injected intraperitoneally into tumor-bearing mice, and the correlation between the near-infrared fluorescence signal at the tumor site and the tumor volume was measured by in vivo imaging. ..

　　Result: Compared with FTS, FTS-IR783 can significantly inhibit the growth of MCF-7, U251 and PC3. Three types of tumor cells can specifically recognize FTS-IR783 and display near-infrared fluorescence aggregation. After subcutaneous injection of tumor model FTS-IR783, in vivo imaging showed that the correlation between the fluorescence intensity and bioluminescence intensity of the tumor site reached 0.987, 0.998 and 0.971, respectively

　　Conclusion: The combination of FTS and the near-infrared fluorescent dye IR-783 can specifically recognize tumor cells and can be used for in vivo imaging of tumor models. At the same time, the compound has tumor-targeting properties and can be significantly inhibited. It is expected to grow tumor cells and become a new type of target drug.