动物造模,肺癌,皮下移植瘤 z-bio 2021-12-30 998

　　Objective: To explore the differences between subcutaneous xenotransplantation of different parts of lung cancer and provide evidence for lung cancer researchers.

　　Method: Mouse Lewis Lewis lung adenocarcinoma cells (ll2-luc-m38) stably expressing luciferase were injected into the right armpit, right inguinal area and foot pad of C57BL/6 mice, and used regularly. The formation and metastasis of subcutaneously transplanted tumors in mice, the survival time and mortality of tumor-bearing mice, the collection of tumor tissues, the pathology of paraffin embedding, sections and HE staining for diagnosis. After the mice were sacrificed, the lung tissue was fixed and metastasis was observed. The subcutaneously transplanted tumor was removed, and the mice survived the surgery and metastasized.

　　Result: Tumors formed early in the axilla and groin, the tumor formation rate was 100%, and the foot pads later formed tumors, the tumor formation rate was 33%. The volume of the inguinal tumor and the axillary group increased rapidly, among which the volume of the inguinal tumor increased the fastest; 21 days after vaccination, 70% of the mice in the axillary group had lung metastases; the number of metastases was high; 50% of the inguinal mice There were lung metastases, but the number of metastases was low; no lung metastases were observed in this group of mice. The mice in the foot pad group had the highest mortality rate. Mice in the groin and axillary groups can undergo subcutaneous tumor resection, and the postoperative survival rate is 100%.

　　Conclusion: In the mouse Lewis lung cancer subcutaneous transplantation tumor model, the inguinal and axillary tumors are high, can tolerate tumor resection, the risk of surgical death is low, easy to monitor, and the operation is simple and reproducible. With a high degree of transferability, the transferability of the next group is excellent.