动物造模,基因突变 z-bio 2021-05-10 583

　　Purpose: In order to enable medical students to understand the principles and techniques of gene mutation detection and effectively improve medical care, we introduce gene mutation animal models into the experimental teaching of "medical genetics" and conduct them in the course of molecular genetics diagnosis. Research and application value of solutions can actually diagnose genetic problems.

　　Methods: Taking 80 undergraduates majoring in basic medicine from Capital Medical University in 2011 and 2012 as the research subjects, and taking hairless mutant mice as the experimental subjects, genomic DNA was extracted from animal tissues, and DNA was extracted, and then molecular genetics were performed. Diagnostic experiment. template. , Use PCR method to amplify target fragments, identify PCR products by electrophoresis, use gel imaging system to analyze the results, and observe the internal connection of the genotype in a genotype animal model with phenotypic characteristics of hairless mice. Based on the results of the experiment, we will design a questionnaire and analyze student feedback.

　　Result: Satisfaction with all indicators in the survey exceeded 90%. 98.7% of students believe that the new experimental teaching model will help them master professional experimental skills, while 96% of students believe in experimental courses. Effectively improve the solution. The ability to ask questions.

　　Conclusion: The use of genetic mutation animal models for molecular genetic diagnosis experiments can effectively improve students' learning effects and cultivate their comprehensive qualities. At the same time, it also provides a reference for how to maximize the use of experimental animal models to expand the educational content and methods of the medical school.