动物造模,异种移植模型 z-bio 2021-05-11 754

　　Objective: To use the PDX model of pancreatic cancer to evaluate the therapeutic effects of clinical chemotherapy drugs and to screen personalized treatment plans.

　　Method: Subcutaneously transplant a fresh surgical specimen of pancreatic cancer into nude mice to establish a PDX model and make it stable for passage. STR genotyping is used to detect the traceability of tumor tissue in the PDX model. Choose oxaliplatin, gemcitabine and iritinib for clinical use. Kang treated three chemotherapeutics and measured the tumor volume. Using a mathematical model of the TGD value, a plasma CA19-9 test was added to evaluate the therapeutic effects of these three chemotherapeutics.

　　Result: The traceability of tumor tissue samples in the PDX model is 99% to 99%, which is consistent with the primary tumor. Compared with the control group, both the irinotecan and gemcitabine groups showed significant therapeutic effects (P = 0.001). Gemcitabine has a more obvious anti-tumor effect; irinotecan has the largest toxic effect, followed by gemcitabine.

　　Conclusion: We have successfully established a PDX model of pancreatic cancer and stable passage. The mathematical model of TGD levels indicates that gemcitabine has the most important role in inhibiting tumor growth.