大气细颗粒物致炎动物模型,动物造模 z-bio 2021-05-11 241

　　OBJECTIVE: To study BALB/c mice by non-invasive tracheal infusion of PM2.5 particle suspension, to explore the pathogenic mechanism of air pollution in animals, and to establish an animal model of air pollution caused by inflammation.

　　Methods: 150 SPF BALB/c mice were divided into blank control group, normal saline group, low PM2.5 group (2.5mg/kg), moderate PM2.5 group (5mg/kg) and high PM2. For the .5 group (10 mg/kg). There were 5 groups in total, and each dose group received tracheal infusion on days 3, 7, 21, 35, and 49. Tissue samples and lung tissues were collected 24 hours after the completion of the tracheal infusion operation. Use ELISA for pathological HE staining. How to verify the feasibility of non-invasive tracheal infusion and whether the inflammation model has been successfully established?

　　Result: Is the success rate of this modeling method 96%? Tracheal infusion was used to simulate the inflammation of lung tissue in mice. The score is positively correlated with airway injection time and dose extension. After exposure to PM2.5, a large number of lymphocytes and macrophages infiltrate the phagocytic particles of the lung, thereby increasing the alveolar space. Compare each exposure group with a normal saline control group. Compared with the blank group, the levels of the inflammatory factor IL-6 in the alveolar lavage fluid and the TNF-α in the lung tissue homogenate increased. Is the difference in the high-dose group the most important?

　　Conclusion: In this experiment, we established mice that caused inflammation through tracheal injection. The model has been successful, which proves that the method is simple and reliable. Is it widely used for repeated infusions in the respiratory system of mice, is it useful for further research on air pollution and other inflammatory mechanisms?