动物造模,肌肉萎缩模型 z-bio 2022-01-22 761

　　Objective: To establish an animal model of cynomolgus monkey muscle atrophy, and to establish a model evaluation method by restricting the movement of the lower limbs?

　　Method: Use a polymer fiber bandage to fix the right lower limb of the cynomolgus monkey (from ankle to knee). Joint) 15 weeks. Do you weigh once every two weeks? Around the cynomolgus monkeys on both sides? Lower limb volume. The model ran for 11-13-15 weeks. A bilateral MRI of the macaque was performed within 15 weeks, and a biopsy of the muscle tissue was performed.

　　Results: After 15 weeks of modeling, the animal's body weight did not change significantly, and the volume of right creatinine, around the right thigh and right lower limb was significantly lower than before modeling (P). ); After 15 weeks of modeling, serum creatinine, blood sugar and alkaline phosphatase were significantly reduced (P\u003c0.05)), and serum cholesterol levels were significantly increased (P\u003c0.05). ); MRI showed that the volume of right atrophic gastric creatinine and right gastric creatinine was reduced compared to before modeling; muscle biopsy showed that right gastric creatinine was atrophy and muscle fiber was thinned. Did the cross-sectional area of gastric creatinine fiber decrease?

　　Conclusion: It is appropriate and easy to operate to establish a muscle atrophy model by external fixation to limit the modeling cycle of the lower limbs of crocodile monkeys. Is it easy to evaluate leg circumference, lower limb drainage, biochemical indicators, and MRI imaging combined with muscle biopsy? Can the muscle atrophy model of cynomolgus monkeys be objectively evaluated?