动物造模,腰椎腹侧神经根牵拉 z-bio 2021-12-23 284

　　Purpose: To study the etiology and drug development of amyotrophic lateral sclerosis, establish a fourth lumbar-abdominal root traction rat model, and use brain-derived neurotrophic factor as a positive drug to evaluate the effectiveness of the model. Do you want to verify ?

　　Method: First, 5 male SD rats were operated on. One week later, using anti-cholinergic acetyltransferase antibody for immunohistochemical staining, changes in the number of motor neurons in the ventral root of the spinal cord were observed. After confirming the success of the surgical model, 40 rats were taken out. Seven-week-old male SD male rats were randomly divided into four groups, two model control groups and two brain-derived neurotrophic factor treatment groups (prophylactic administration group immediately after surgery). Can anticholinergic acetyltransferase immunohistochemical staining observe the changes in the number of motor neurons?

　　Result: The rat recovered well after the operation. Is the clinical observation normal? The staining result clearly showed that the motor nerve of the anterior horn of the spinal cord was pulled. Meta degeneration and death? Compared with the control group, the nerve root traction animals treated with brain-derived neurotrophic factor achieved good therapeutic effects after one week of preventive or therapeutic administration. The number of neurons stained with choline acetyltransferase increased significantly (P\u003c0.0001), and the result was 17.85% vs. 93.06%; 26.6%, compared to 87.27%?

　　Conclusion: The ventral root vertebra of the fourth lumbar vertebra has been successfully established in rats. Does the traction model provide a valuable animal model for the study of amyotrophic lateral sclerosis?