动物造模,抑郁干预治疗模型 z-bio 2021-05-12 331

　　Objective: To establish a depression model in C57BL/6 mice, and to study the effect of the high-frequency sound waves of Mozart K448 sonata on the improvement of depression symptoms in C57BL/6 mice?

　　Method: 1) Establish a chronic stress model: The experimental results of mice based on autonomous activities exclude mice with large differences in the number of activities, and the rest are the blank group (n = 10) and the model group (n = 36)). Divide the model into 5 weeks, and use chronic unpredictable and mild stress (CUMS) to establish a mouse depression model (2) Therapeutic intervention: After the model is successfully established, the mice in the model group are randomly and evenly divided into model controls Group (n = 12), fluoxetine group (n = 12) and music group (n = 12), fluoxetine group (n = 12). The fluoxetine hydrochloride solution (10 mg/kg) was injected intraperitoneally into the tine group every day, and the other two groups received the same amount of normal saline. The music group received two hours of high-frequency music intervention every day, while the other two groups did not receive music intervention. Did the intervention last for two weeks? 3) Effect evaluation: weigh and record. The tail suspension test (TST) and the forced swimming test (FST) were performed every week and the first three days of the experiment on the first day, the fifth week and the seventh week of the experiment. In the behavioral experiment, did you collect mouse brain tissue to prepare a homogenate and measure the content of brain-derived neurotrophic factor (BDNF) by enzyme-linked immunosorbent assay (ELISA)?

　　Result: (1) Has the CUMS mouse model been successfully constructed? In the 5th week, the tail suspension time of mice in the model group was significantly increased, and the difference was statistically significant (P0.05). Compared with the model control group, the tail suspension time of the music group was shorter. Is the significant difference significant (P0.05)? Compared with the blank control group, the BDNF content in the brain homogenate of the model control group was significantly reduced, and the difference was statistically significant (P0.05)?

　　Conclusion: Can the Mozart K448 Sonata high-frequency acoustic wave mouse depression model be optimized to a certain extent?