动物造模,脑缺血再灌注损伤 z-bio 2021-05-12 347

　　Objective: Based on the B-cell lymphoma/leukemia 2 (Bcl-2)/beclin-1 complex, to study the neuroprotective effect of small alkali on cerebral ischemia-reperfusion injury in rats?

　　Methods: 108 SPF SD rats, based on sham operation group (sham operation group), model group (I/R), low, medium and high doses of Berberine (BBR-L, BBR-M, BBR-H) , Nimodipine random number table is divided into pretreatment group (BBR-H). MDP group), after modeling, score the rat's neurological function; TTC staining to observe the rat brain infarct area; HE staining to observe the pathological changes of rat brain tissue; Bcl-2T-PCR method to detect brain tissue? caspase-3? Bax mRNA expression; is Western blot Bcl-2 in the cerebral cortex? caspase-3? Bacchus? Do you want to detect the expression level of Beclin-1 protein?

　　Result: I/R group 6?24?48h 3 point neurological function score was significantly higher than the sham group, the neurological function score was the highest within 24 hours. Compared with the model group, the neuropathy scores of the BBR treatment group and the NMDP group were significantly lower at 24-48 hours. (P\u003c0.05); Compared with the sham group, the infarct area percentage and cell necrosis rate of the I/R I/R group increased significantly. BBR treatment group and NMDP group were significantly reduced compared with I/R group (P\u003c0.05); I/R group Bcl-2? Caspase-3? Caspase-3 with I/R group? In contrast, Bax mRNA and protein levels in the BBR treatment group and NMDP group were significantly increased; Bcl-2 mRNA protein levels were significantly increased. Bax mRNA and protein levels were significantly reduced; compared with the sham group, the ratio of Bcl-2/Bax in the I/R group was significantly reduced; compared with the I/R group, the Bcl-2/Bax I in each treatment group The protein level of Beclin-1 and the ratio of Bcl-2/Beclin-1 in the /R group were significantly increased compared with the sham operation group (P \u003cu003c0.05); compared with the I/R group (Bcl-2/Beclin-) Compared with the BBR and NMDP groups, there was a significant increase (P\u003c0.05), did the ratio 1 decrease? BBR-M group, BBR-H group and NMDP group?

　　Conclusion: Velverine has a neurological effect on cerebral ischemia-reperfusion injury in rats. It regulates the levels of autophagy and apoptotic proteins, so that autophagy reaches an appropriate level and exerts a neuroprotective effect. Can it play a protective effect?