动物造模,精神分裂症模型 z-bio 2021-12-15 464

　　Objective: To establish a rat model of schizophrenia, observe the pathological damage of the prefrontal cortex and the expression changes of PKA and endothelial cell chemokine-5, clarify the mechanism of nerve damage, and guide clinical treatment. Does it provide you with a reference?

　　Methods: The main points of the experiment were randomly divided into two groups: the control group (normally raised rats, subcutaneously injected with saline, n = 15) and the induction group (intraperitoneal injection of 0.5 mg/kg of amphetamine per day to establish a schizophrenia model, n = 15) Using successful hematoxylin-eosin staining, the nuclear contraction of the frontal field neurons in the rat was detected. Using the Sams-Dodd stereotyped behavior scoring system and field experiments, the rat model of schizophrenia was tested. T detects the expression of PKA and CCL5 mRNA. -qRCR; Use enzyme-linked immunosorbent assay kit method to detect the protein levels of PKA and CCL5 in experimental rats; detect the protein expression levels of IL-1α, IL-1β and IL-17 by Western blotting.

　　Result: The stereotype score of rats in the induction group (0.85±0.14) was increased (2.38±0.26) compared with the control group, and the open field experimental induction group (326.58±15.47) was higher than the control group (198.55). ), the price is also very expensive. ±12.58) (P\u003c0.05); Compared with the control group, the expression levels of PKA and CCL5 mRNA in the induced group increased (P\u003c0.05); PKA (4.21±1.05) mmol/g and CCL5±0.51) (3.76 ) The level of mmol/g in the induction group was higher than that of the control group (2.46±0.67) mmol/gg, and (1.35±0.24) mmol/g was higher than the control group (P\u003c0.05); about protein expression of inflammatory factors (IL-1α) ?IL-1β?IL-17), induction group (2.85±0.35)? 2.15±0.27)? (2.16±0.32) and the control group (1.02±0.17)? (0.94±0.13)? (1.05±0.25) increased (P\u003c0.05); the proportion of positive cells in the induction group was higher than that of the control group, the nuclei of frontal vertebrae contracted a lot, and the cytoplasm of eosinophils stained increased (P\u003c0.05). u003c0.05)?

　　Conclusion: Does the increased expression of PKA and CCL5 in the prefrontal cortex of the schizophrenia rat model cause cognitive impairment?