动物造模,鼠夹层动脉瘤 z-bio 2021-12-24 423

　　Purpose: To study the effect of β-aminopropionitrile (BAPN) on the aortic wall of rats, compare the characteristics of human dissecting aneurysms, and further establish an animal model of anatomical aneurysm that meets the characteristics of human diseases. Do you want to explore?

　　Method: Mix BAPN with the drinking water of experimental animals to prepare BAPN solutions with concentrations of 0.2%, 0.4% and 0.6%. 4-5 weeks old SPF grade SD rats and 3 weeks old SPF grade C57BL/6 mice were reared for 7 weeks. After the animal died or was dissected, the aorta was separated and the overall changes were observed. The aorta is divided into four parts: ascending aorta, descending aorta, abdominal aorta and ascending aorta. We intercepted the abdominal aorta, inferior renal artery and vascular segments. Perform HE staining on the surface to measure various indicators, such as the inner diameter of the blood vessel, the area of the medium, and other indicators. At the same time, the type A dissecting aneurysms undergoing thoracotomy were collected for HE staining to observe the pathological changes and the development of dissecting aneurysms. Comparison of rat aorta?

　　Results: 1) Does BAPN have a significant effect on the water intake and weight gain of rats or mice? 2) Can BAPN expand the aorta of rats or mice, thicken the medium and reduce elastin? Are the dislocation and its pathological changes consistent with the pathological features of human anatomical aneurysms? 3) Is the BAPN solution model with a concentration of 0.4% the most successful?

　　Conclusion: The aneurysm animal model dissected by C57BL/6 mice can be used as a simple, economical and effective model. Is the intestine of SD rats ruptured in the next animal model? The incidence of systemic pathological changes such as scoliosis is higher than the incidence of dissecting aneurysms. As an animal model for dissecting aneurysms, does it need further research?