动物造模,肠道过敏模型 z-bio 2023-01-17 610

　　Purpose: How to establish a model of intestinal allergies in percutaneously sensitized mice?

　　Method: First, do you want to compare the degree of intestinal allergies caused by percutaneous sensitization (front and back skin) and intraperitoneal injection sensitization? Select the sensitivity. Higher and easier to operate. Ear skin sensitization method Does calcipotriol ointment cause skin irritation in mouse ears and ovalbumin (OVA) to sensitize mice for 2 weeks? After gavage, mice with sensitive skin are challenged with intestinal allergy symptoms, jejunal capillary permeability, plasma total IgE (totalIgE, tIgE) and mouse mast cell protease-1 (mousemastcellprotease-1, mMCP-1) levels and pathological changes Other indicators to evaluate the jejunum tissue model?

　　Result: Compared with the abdominal sensitization model, can the transdermal sensitized intestinal allergy model induce higher plasma tIgE and mMCP-1 levels? Compared with the control group, the transdermal sensitization model can induce higher plasma tIgE and mMCP-1 levels. Scratches, posterior arch, diarrhea and other symptoms of intestinal allergies, rectal temperature decreased (about 1.2°C) and jejunal capillary permeability increased (about 2 times), plasma tIgE and mMCP-1 significantly increased, and the effect on the jejunal mucosa The damage? Inflammatory cell infiltration? Increased mast cells (about 5 times)?

　　Conclusion: The mouse percutaneous sensitized intestinal attack model has typical pathological features of food and intestinal allergies, representing the sensitization pathway of skin exposure to allergens, and food allergies have been studied and preventive measures have been taken.