动物造模,高尿酸血症模型 z-bio 2021-10-09 834

　　Objective: To establish a rat model of hyperuricemia to explore whether hyperuricemia can cause secondary cardiovascular disease.

　　Method: 32 SPF male SD rats were randomly divided into normal control group (GroupC), potassium oxalate model group (GroupM1), potassium oxalate and high-sugar and high-fat diet model group (GroupM2), and oxazine potassium acid group. In the yeast extract feed model group (GroupM3), there are 8 animals in each group, and the model is continuously created for 3 weeks. By comparing rat serum uric acid (UA), blood uric acid (BUN), creatinine (Cr), insulin (insulin, INS), blood sugar (GLU), triglycerides (TG) and other indicators of hyperuricemia. Study this disease in a rat model.

　　Results: Hyperuricemia model rats were replicated by gavage of 750 mg/kg body weight of potassium oxalate and yeast extract diet, showing a significant increase in UA levels (P\u003c0.01), and long-term maintenance of pathological changes showed that Over time 3/8-year-old children can cause hyperuricemia, but the changes of GLU, INS, TG levels in the heart of 3/8 rats and the pathological changes of the heart also follow, which suggests secondary heart The occurrence of vascular disease.

　　Conclusion: The combined modeling method of oxazine potassium and yeast paste diet is more suitable than simple oxazine potassium to establish a rat model of long-term hyperuricemia, accompanied by secondary blood glucose disorders. The model construction program can be used as an animal model for studying the intervention mechanism of hyperuricemia and cardiovascular disease, and can be used for comprehensive evaluation of the preclinical pharmacodynamics of hyperuricemia therapeutic agents.