动物模型 z-bo 2020-08-06 569

　　(1) Copy method Experimental rabbits are fixed after routine anesthesia, and one side of femoral artery and vein are separated. 20ml blood was collected through femoral vein cannulation, 51Cr labeled platelets. Inject the labeled platelets from the ear vein and insert the balloon catheter through the femoral vein immediately. When the tip of the catheter reaches the junction of the thoracic and abdominal aorta, the balloon is inflated to 600mmHg (80kPa), the balloon tube is pulled down 10cm, and then re-inserted to the junction after deflation Place, inflate again, pull down, and repeat 6 times to remove the catheter, ligate the artery, and suture the incision. Animals were killed by intravenous injection of overdose of sodium pentobarbital 1 hour after the intimal membrane was removed. Before sacrifice, 5 ml of 0.5% Evans blue and 1000 U of heparin were injected intravenously. Separate the aorta, intercept the upper 5cm thoracic aorta and the lower 10cm uniformly blue-stained abdominal aorta, rinse them twice in normal saline, count the counts per minute (CPM value) of each arterial segment, and weigh it after sufficient drying, and calculate The number of platelets marked on the artery segment per gram of dry weight.

　　(2) Features of the model The balloon tube is used to injure the intima to form an abdominal aortic thrombosis model in rabbits. The method is reliable, simple and easy to implement, with small subjective factors and quantitative analysis.

　　(3) Comparative medicine. When the balloon tube damages the intima to produce arterial thrombosis, the media is also damaged to varying degrees. However, the arterial thrombus caused by intimal injury in clinic is only confined to the surface, which is more severe than the clinical damage, but the media is damaged. The nature of the thrombus formed is consistent with the arterial thrombus formed by clinical intimal injury, and does not affect the observation of drug efficacy.